CHEM 133 Week 8 Lab 8 Assignment

 CHEM 133 Week 8 Lab 8 Assignment | American Public University System

Question 1 of 9

Please upload (in PDF format) a picture of your three before analyte preparation beakers with their contents (Step 4). To receive credit, the beakers and their contents must be clearly visible in the picture, as well as clearly labeled with your name, the lab title (with “before analyte preparation” indicated), and the date in clear view. This must be a single PDF file in order to receive credit.

 

Question 2 of 9

Please upload (in PDF format) a picture of your prepared analyte experimental setup (Step 5). To receive credit, your beaker, your solvent (mobile phase), and your filter paper (stationary phase) with spots (analyte) and initial pencil-marked spotting line should all be clearly visible, and also include in your picture a label with your name, the lab title (with “prepared analyte” indicated), and the date in clear view. This must be a single PDF file in order to receive credit.

NOTE: If your document is not clear, organized, and properly labeled, or any part of it is unclear to your instructor, you will not receive credit.

 

 

 

 

Question 3 of 9

Please upload (in PDF format) a picture of your 4 chromatograms (Step 6). To receive credit, all solvent names, Rf values, and color labels should all be clearly visible. Also include in your picture a label with your name, the lab title (with “analyte results” indicated), and the date in clear view. This must be a single picture in a single PDF file in order to receive credit.

NOTE: If your document is not clear, organized, and properly labeled, or any part of it is unclear to your instructor, you will not receive credit.

 

 

 

 

Question 4 of 9

Please upload (in PDF format) your completed Table 1: Paper Chromatography Data and Observations and Rf Calculations (Step 7). To receive credit, these must be neatly organized and labeled and together in a single PDF document.

 

 

 

 

Question 5 of 9

According to the experimental results, which solvent provided the best separation?

 

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A. 0.5% NaCl solution

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B. all solvents provided the same degree of separation

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C. 70% isopropyl alcohol

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D. 0.2% NaCl solution

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E. Distilled Water

 

Question 6 of 9

Based on the experimental results, which solvent provided the SECOND best separation of the analyte?

 

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A. 70% isopropyl alcohol

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B. all solvents had the same degree of separation

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C. vinegar

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D. 0.2% NaCl solution

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E. 0.5% NaCl solution •   

F. distilled water

 

Question 7 of 9

4.0/ 4.0 Points

Some children have reactions to Yellow 5 Dye or Lake. Yellow 5 is a pale yellow color and Yellow 6 is more orange. Look at your green, red, and blue spots. Determine by the colors seen on the chromatograms for these 3 colors, which of these colors of M & M candies you tested contain Yellow 5.

 

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A. red

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B. green

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C. green, red, and blue all contain Yellow 5

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D. blue

 

Question 8 of 9

Traditionally in chromatography the eluting solvent is an organic compound, such as hexane or acetonitrile. Explain, in terms of green chemistry, why this experiment uses environmentally friendly compounds, such as table salt, water and isopropyl alcohol.

 

Research an alternative solvent that is currently being used in chromatography to reduce costly wastes and describe the benefits of that solvent, as well as any complications that may be related to the use of the alternative solvent.

 

Your answer must be clear and complete (with references) to receive credit.

 

The more environmentally friendly a solvent is, the better it is for everyone, in short. However, there is more to this then just protecting one area, and as such, these green chemistry agents are easier to break down and utilize in more than one way, so there can be many uses for them, rather than one typical standard use. By using these solvents, it cuts down on environmental wastes.

Scientist are now using a green liquid form of chromatography in the mobile field, which creates a greener and more waste-less approach. This process now uses cyclodextrins as additives. By using the cyclodextrins as additives, it allows more water usage in chemistry wastes, so it breaks down at a much better rate, thereby reducing the overall environmental impact. "Cyclodextrins are a natural, renewable compound made from starch and are totally biodegradable" (Too, K., 2010) With this new process in chemistry waste, the outcome will be greener.

 

 

 

Question 9 of 9

Answer the following Post-Lab Questions on potential sources of error in this experiment.

1) What could have or did go wrong?

2) How might this have impacted your data?

3) How could you address/fix such errors in future experiments?